description
Human NOTCH1 was cloned as a chromosome 9 gene, translocated to the T-cell beta receptor (TCBR) promoter on chromosome 7 in T-cell acute lymphoblastic leukemia (T-ALL) (Ellisen et al. 1991). This translocation, present in only a small percentage of T-ALL patients, results in the overexpression of a truncated NOTCH1 receptor, which lacks almost the entire extracellular domain, in T lymphocytes. Oncogenic NOTCH1 mutations were subsequently found to be present in >50% of T-ALL patients, with hotspots in the heterodimerization domain (HD domain) and PEST domain of NOTCH1 (Weng et al. 2004). Normal NOTCH1 becomes activated by binding DLL (DLL1 or DLL4) or JAG (JAG1 or JAG2) ligands expressed on the surface of a neighboring cell, which leads to proteolytic cleavage of NOTCH1 by ADAM10/17 and gamma-secretase, and release of the NOTCH1 intracellular domain (NICD1) which regulates expression of genes that play important roles in the development of T lymphocytes (Washburn et al. 1997. Radtke et al. 1999, Maillard et al. 2004, Sambandam et al. 2005, Tan et al. 2005). Mutations in the HD domain, responsible for association of NOTCH1 extracellular and transmembrane regions after furin-mediated cleavage of NOTCH1 precursor, as well as the truncation of the NOTCH1 extracellular domain by the rare T-ALL translocation, enable constitutive production of NICD1, in the absence of ligand binding (Malecki et al. 2006, Ellisen et al. 1991).Mutations in the NOTCH1 PEST domain interfere with FBXW7 (FBW7)-mediated ubiquitination and degradation of NICD1, resulting in prolonged half-life and increased transcriptional activity of NICD1, which promotes growth and division of T-lymphocytes (Weng et al. 2004, Thompson et al. 2007, O'Neil et al. 2007).Mutations in the HD domain and PEST domain of NOTCH1 are frequently found in cis in T-ALL. While HD mutations alone result in up to ~10-fold increase in NOTCH1 transcriptional activity and PEST domain mutations alone result in up to ~2-fold increase in NOTCH1 transcriptional activity, in cis mutations of HD and PEST domains act synergistically, increasing NOTCH1 transcriptional activity up to ~40-fold (Weng et al. 2004).FBXW7 (FBW7), a component of the SCF (SKP1, CUL1, and F-box protein) ubiquitin ligase complex SCF-FBW7 involved in the degradation of NOTCH1 (Oberg et al. 2001, Wu et al. 2001, Fryer et al. 2004), is subject to loss of function mutations in T-ALL (Akhoondi et al. 2007, Thompson et al. 2007, O'Neil et al. 2007) which are mutually exclusive with NOTCH1 PEST domain mutations (Thompson et al. 2007, O'Neil et al. 2007).Although gamma-secretase inhibitors (GSIs) are successfully used in vitro to inhibit NOTCH1 signaling in T-ALL cell lines, the gamma-secretase complex has many other substrates besides NOTCH. The specificity of GSIs is therefore limited and, as they are not considered to be particularly promising drugs for the clinical treatment of T-ALL (reviewed by Purow, 2012), they have not been annotated.For a recent review of NOTCH1 signaling in cancer, please refer to Grabher et al. 2006

external resources
NCBI:1268883
REACTOME:R-HSA-2644603
PUBMED:17646409
PUBMED:10367900
PUBMED:16738328
PUBMED:15951812
PUBMED:22399357
PUBMED:15546612
PUBMED:11461910
PUBMED:9118226
PUBMED:15951813
PUBMED:17646408
PUBMED:15472075
PUBMED:11585921
PUBMED:17909001
PUBMED:16612405
PUBMED:1831692
PUBMED:15187027

genes
ADAM10 , JAG1 , CCNC , CDK8 , CREBBP , EP300 , KAT2A , HDAC1 , HDAC2 , HES1 , RBPJ , JAG2 , MYC , NOTCH1 , PSEN2 , RPS27A , SKP1 , ADAM17 , TBL1X , UBA52 , UBB , UBC , CUL1 , HDAC3 , KAT2B , NEURL1 , NCOR1 , NCOR2 , HDAC9 , HDAC4 , MAML1 , RBX1 , HDAC6 , HDAC5 , MAMLD1 , SNW1 , NCSTN , HEY1 , HEY2 , HEYL , DLL1 , APH1A , HDAC7 , NEURL1B , DLL4 , FBXW7 , MAML3 , PSENEN , HDAC8 , MIB1 , TBL1XR1 , HDAC11 , APH1B , HDAC10 , MAML2 , MIB2 , HES5 , MIR1281 ,