description
Small interfering RNAs (siRNAs) are 21-25 nucleotide single-stranded RNAs produced by cleavage of longer double-stranded RNAs by the enzyme DICER1 within the RISC loading complex containing DICER1, an Argonaute protein, and either TARBP2 or PRKRA (PACT). Typically the long double-stranded substrates originate from viruses or repetitive elements in the genome and the two strands of the substrate are exactly complementary.After cleavage by DICER1 the 21-25 nucleotide double-stranded product is loaded into an Argonuate protein (humans contain 4 Argonautes) and rendered single-stranded by a mechanism that is not well characterized.siRNA-loaded AGO2 is predominantly located at the cytosolic face of the rough endoplasmic reticulum and has also been observed in the nucleus

external resources
NCBI:1269721
REACTOME:R-HSA-426486
PUBMED:19165215
PUBMED:24388755
PUBMED:15741316
PUBMED:19239886
PUBMED:23511973

genes
TARBP2 , TSN , TSNAX , PRKRA , DICER1 , AGO1 , AGO2 , AGO3 , AGO4 ,